A new paper titled ‘Probabilistic cell seeding and non-autofluorescent 3D-printed structures as scalable approach for multi-level co-culture modeling‘ was published in Materials Today Bio today.
In this work, we have developed a 3D-printed platform that enables precise control and in-depth study of the interaction between astrocytes and neurons. To achieve this, we utilized a commercially available printing resin IP-Visio (Nanoscribe), with non-autofluorescent and non-cytotoxic properties. We successfully printed a neurite guidance platform, which served as a guiding structure for the outgrowth of neurites from neurons. By employing a probabilistic two-step cell seeding approach, we were then able to control the interaction points between neurons and astrocytes. This innovative approach simplifies the modeling of complex brain interactions, thereby facilitating further advancements in biomedical research and enhancing our understanding of the human brain.