New paper published in ACS Applied Materials & Interfaces

The article “Sorption of Neuropsychopharmaca in Microfluidic Materials for In Vitro Studies” was published in ACS Applied Materials & Interfaces today. The study highlights the impact of peristaltic pump tubing in sorption of hydrophobic compounds. mainly consisting of neuropsychopharmaca. The article further displays that the use of PDMS or other device construction methods OSTE+ or PC/PSA had a similar effect on the sorption, whereas the material of the tubing had a stronger dependence on sorption as compared to the device material. This signifies that the tubing and associated materials deserve similar attention as other device materials used for in-vitro studies.

link to the article

New Postdoctoral scholar joins the group

Dr. Rohollah Nasiri joins the Herland group as our newest postdoctoral scholar. He received his PhD and MSc degrees in Mechanical Engineering from the Sharif University of Technology, Iran, in 2021 and 2014, respectively. His research focusses on designing organ-on-a-chip devices integrated with biosensors for disease modeling and drug screening applications.

New paper published in the Journal of Materials Chemistry C

The article “Rapid prototyping of heterostructured organic microelectronics using wax printing, filtration, and transfer” was published in the Journal of Materials Chemistry C today. The research highlights rapid prototyping of various micropatterned organic electronic heterostructures of PEDOT:PSS using hydrogels filtered onto membranes containing hydrophobic wax patterns. The article also demonstrates the potential of this method for micro-supercapacitors, organic electronic transistors, and their use in cell culture to enable bioelectronics.

link to the article

New paper published in Small

The article “Continuous Monitoring Reveals Protective Effects of N-Acetylcysteine Amide on an Isogenic Microphysiological Model of the Neurovascular Unit” was published in Small today. The article reports a microphysiological blood-brain barrier model that captures the multicellular interactions of iPS-derived cells. The integrated electrical sensors, facilitated by PDMS-free fabrication, allow for real-time monitoring of how the barrier responds to oxidative stress and antioxidant prophylaxis. The sensor integrated hiBBB-on-chip displayed an immediate utility in the screening of drugs modulating the barrier by providing readout about the temporal pharmacodynamic profiles.

The article was also featured on the inside Back cover of the journal.

link to the article

link to the cover

Inside Back cover of SMALL

Open Post doc position at Herland Lab

We have an open Postdoc (scholarship) position in the Wallenberg foundation funded project Organs-on-Chips for Translational Research in Brain Disease. This project will be focusing on inborn errors of metabolism in children, combining neural models with real-time sensing of neural function, barrier function and metabolic activity. Conventional cell culture, as well as microfluidic Organ-on-Chip methods will be applied. The post doc scholarship will focus on developing functional stem-cell derived cells for modelling inborn errors of metabolism combined with Brain-on-Chip systems. The postdoc will work closely together with engineers in the Herland lab and clinical researchers at Karolinska Hospital. More details can be found here.

Link to the advertisement on KTH webpage

New Article published in International Journal of Molecular Sciences

The article “Bisphenol A Inhibits the Transporter Function of the Blood-Brain Barrier by Directly Interacting with the ABC Transporter Breast Cancer Resistance Protein (BCRP)” was published in the International Journal of Molecular Sciences today. The article highlights that bisphenol A (BPA), a widely used chemical in consumer products affects the transport function of breast cancer resistance protein (BCRP). This effect is suggestive to BPA’s known detrimental impact on neurodevelopment.

link to the article on MDPI

New Article Published in Nature Physics

The article “Fluid interfacial energy drives the emergence of three-dimensional periodic structures in micropillar scaffolds” was published in Nature Physics today. The article highlights an elegant design and building approach of periodic three-dimensional multi-material fluid lattice architectures which can also be used for composites, droplet networks and can also support the growth of biological cells and tissues.

link to the article in Nature Physics

link to the news highlight on the article

Research featured in ATLA (Alternatives to Laboratory Animals)

Our recent research article titled ‘Low-cost microphysiological systems: feasibility study of a tape-based barrier-on-chip for small intestine modeling‘ which was published in Lab on a Chip earlier this year, was featured in the News and Views section of the journal ATLA (Alternatives to Laboratory Animals).

Organs-on-Chips will be critical to reducing animal experiments in the future. There are many challenges still to overcome toward their widespread use, one important challenge being the high cost and complexity of manufacturing current systems, both commercially or in an academic setting. In our work, we showcase a functional Barrier-on-Chip of the small intestine, fabricated using very simple and low-cost methods that practically anyone can implement in their lab, but that can also be adapted to large-scale industrial manufacture. We hope that this can make Barrier-on-Chip technology much more broadly accessible, particularly to researchers and prototypers in low-resource environments.

Comments from Prof. Anna Herland on the feature in ATLA

Link to the feature in ATLA (Alternatives to Laboratory Animals)

Link to the original research article in Lab on a Chip

New Master’s student joins the group

Violetta Nikiforova joins the Herland group as our newest master’s student. She is currently enrolled in the Master’s programme in Toxicology at Karolinska Institutet. For her thesis, she will be working with Xenia on the development and validation of novel in-vitro models for adverse effects on the human neurovascular unit.

New Master’s students join the group

Today, Saskia Ludwig and Eleni Stergiou join the Herland group as our newest Master’s students. Saskia is pursuing a Master’s degree in Fundamental Neuroscience at Maastricht University in the Netherlands and will work on her thesis with Isabelle on spontaneous differentiation of neuroepithelial stem cells in 3D hydrogels at KTH. Eleni is currently pursuing a Master’s degree in Molecular Medicine at Uppsala University and will work on her thesis with Xenia on the development of hiPSC-based in-vitro models of neurovascular unit for screening and investigation of novel drug modalities.

New review article published in Brain

An extensive review article “Recent progress in translational engineered in vitro models of the central nervous system” was published in Brain today. Together with Ben M.Maoz and his group at the Tel Aviv University, we discuss the recent developments in in-vitro CNS models with focus on combined microfluidics and cell culture systems (e.g. ‘organ-on-a-chip’ systems). The article also highlights the challenges hindering the advancement of this field and its large-scale acceptance and implementation and underlines some practical guidelines for labs venturing into this field.

link to the article

New perspective article published in Nature

The perspective article “LifeTime and improving European healthcare through cell-based interceptive medicine” was published in Nature today. The LifeTime Initiative consists of experts from varied disciplines from over ninety research institutes from all over the EU. The initiative aims to develop and integrate novel technologies such as patient-derived experimental disease models, single-cell multi-omics, high content imaging and artificial intelligence to address the current medical challenges and incorporate them into regular laboratory and clinical workflows resulting in a great benefit for healthcare providers and patients alike.

link to the article on Nature

New paper published in Advanced Biosystems

The article “Proteomic and Metabolomic Characterization of Human Neurovascular Unit Cells in Response to Methamphetamine” was published in the Advanced Biosystems today. The research highlights the effect of methamphetamine on the proteomic and metabolomic properties of constituent cells of the neurovascular unit, especially those of human pericytes not reported previously. This work presents new pathways that are active in the cells at homeostasis and as a response to Meth, which could be important when studying the detrimental effects of Meth intoxication on the CNS.

link to the article in Advanced Biosystems

New review article published in Molecular and Cellular Neuroscience

The review article “Models of the blood-brain barrier using iPSC-derived cells” was published in Molecular and Cellular Neuroscience today. The article discusses the capability for the use of human iPSC- derived brain cells for modelling the blood-brain barrier (BBB) over the conventionally used immortalized brain endothelial cell lines and primary brain endothelial cells of human and animal origin. The review highlights the essential functions of the BBB and the ongoing efforts in the creation of iPSC-derived BBB models along with the key requirements and challenges faced currently.

link to the article in Molecular and Cellular Neuroscience

New paper published in Micromachines

The article “Low-Cost PVD Shadow Masks with Submillimeter Resolution from Laser-Cut Paper” was published in Micromachines today. The research highlights an affordable method to produce shadow masks for physical vapor deposition using cellulose filter paper and CO2 laser processing. These masks are shown to be stable and resilient in processing and handling. This method holds a lot of potential for a range of applications such as electrochemical cells and contact pads for organic electronic materials where submillimeter PVD features are required.

Link to the original article at Micromachines

New paper published in ACS Omega

The article “Electrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe” was published in the ACS Omega today. The research illustrates a method to integrate isothermal DNA amplification, recombinase polymerase amplification to an electrochemical stem-loop probe DNA detection technique while reducing the need for purification of the amplified product and improving the sensitivity and limit of detection.

link to the article in ACS Omega

New paper published in the Journal of Colloid and Interface Science

The article “Bactericidal surfaces prepared by femtosecond laser patterning and layer-by-layer polyelectrolyte coating” was published in the Journal of Colloid and Interface Science today. The research focusses on the modification of borosilicate glass surfaces through µm-scale patterning using ultrashort pulsed laser irradiation and a layer-by-layer polyelectrolyte modification of the surface with charge values at least 10 times lower than the previously reported cationic bactericidal surfaces. This method yields enhanced bactericidal effect against both Gram-positive and Gram-negative bacteria, holding immense potential in the field of hygiene products and medical devices.

Link to the article in the Journal of Colloid and Interface Science

New paper published in Advanced Biosystems

The article “hiPS‐Derived Astroglia Model Show Temporal Transcriptomic Profile Related to Human Neural Development and Glia Competence Acquisition of a Maturing Astrocytic Identity” was published in the Advanced Biosystems today. The research highlights the directed differentiation of neuroepithelial stem cells undergoing a neurogenic-to-gliogenic competence. This NES-Astro model can be used for detailed mechanistic studies of RG and astrocyte development under normal conditions in addition to disease models.

link to the article in Advanced Biosystems

New paper published in Lab on a Chip

The article “Low-cost microphysiological systems: Feasibility study of a tape-based barrier-on-chip system for small intestine modeling” was published in Lab on a Chip today. The research highlights the use of simple double-sided tape to assemble an organ-on-a-chip system with human gut as a model, replacing the typically used expensive techniques and materials. This method will prove to be a robust and reproducible approach to studying physiological barriers, promising faster development of new drugs and precision and personalized medicine.

Link to the article in Lab on a Chip

Link to KTH Press Release

Link to the back cover in Lab on a Chip

Back cover, Lab Chip, 2020,20

New paper published in the Journal of Visualized Experiments (JoVE)

The article “Generation of a Human iPSC-Based Blood-Brain Barrier Chip” was published in the Journal of Visualized Experiments (JoVE) today. The research illustrates the possibility to combine organ-on-chip and induced pluripotent stem cell (iPSC) technologies to generate a BBB chip fully personalized to human barrier formation on a functional and transcriptional level.

Generation of a Human iPSC-Based Blood-Brain Barrier Chip

Two new papers in Nature Biomedical Engineering

Two new articles on multi-organ in-vitro models developed in collaboration with the Wyss Institute were just published in Nature Biomedical Engineering as part of a collection on Microphysiological Systems: “Quantitative prediction of human pharmacokinetic responses to drugs via fluidically coupled vascularized organ chips” and “Robotic fluidic coupling and interrogation of multiple vascularized organ chips“. These platforms could drastically accelerate drug testing and provide accurate predictions of drug effects prior to clinical testing.

link to KTH press release

link to Wyss Institute press release

Thomas at the European Research and Innovation Days

Thomas pitched his (and the lab’s) research at the Marie Curie Falling Walls Lab in Brussels as part of the European Research and Innovation Days. He and the other 29 participants (selected from over 200 applicants) got the opportunity to present their research to a broad audience – in under 3 minutes each.

If you missed the live stream, you can watch his pitch here. The video is courtesy of the European Commission.

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New PhD students join the group

Over the summer, Saumey Jain and Sebastian Buchmann have joined the Herland group as our newest PhD students. Saumey, who received his M.Sc. from KTH, is co-supervised by Prof. Frank Niklaus and will be working on nanopore sequencing. Sebastian, who graduated from the University of Basel, is co-supervised by Prof. Max Hamedi and will be working on organic electronic sensing and stimulation.

Herland lab at EurOoC in Graz

The KTH team spent two engaging & enjoyable days in Graz, Austria, at the European Organ-on-Chip conference.

Isabelle & Dimitris presented posters with their latest research results. Dimitris won one of four Travel Grants with his poster.

Thomas gave the Highlighted Presentation in the session on “Materials, analytics and in-line sensing”. His talk was awarded Best Paper at the closing ceremony.

Prof. Herland gives Keynote lecture

New Review published in Advanced Materials

Our expansive review paper “Conjugated Polymers for Assessing and Controlling Biological Functions” was published in Advanced Materials today. The article will be part of an issue dedicated to Professor Olle Inganäs (Linköping University) on the occasion of his Professor Emeritus title. Together with A.L. Rutz and G.G. Malliaras at the University of Cambridge, we discuss the use of conjugated polymer materials in five biologically oriented research topics: electrophysiology, tissue engineering, drug release, biosensing, and molecular bioelectronics.

link to the original article at wiley.com

New Paper published in Nature Biotechnology

The article “A linked organ-on-chip model of the human neurovascular unit reveals the metabolic coupling of endothelial and neuronal cells” was published in Nature Neuroscience today. The research illustrates how fluidically linked Blood-Brain Barrier and Brain Organ Chips offer new methods for studying the effects of drugs and disease on the brain and its blood vessels.

link to press release at the Wyss Institute

link to coverage at motherboard.com

link to the original article at nature.com

New intern joins the group

Today, Simon Feillée joins the Herland group as our newest internship student. He is pursuing a degree at Mines Paristech in France, and for her internship project will be working with Isabelle and Thomas on the controlling the surface chemistry of a novel polymer for microfluidic device fabrication.

New ERASMUS+ intern joins the group

Today, Eva Stronkman joins the Herland group as our newest ERASMUS+ internship student. She is pursuing a degree at Saxion University of Applied Sciences in the Netherlands, and for her internship project will be working with Thomas on the novel device fabrication methods for brain on a chip devices.

New Master’s student joins the group

Today, Dimitri Wirjowerdojo joins the Herland group as our newest Master’s student. He is getting his degree in Molecular Techniques in Life Science, and for his thesis work will be working with Dimitrios on the evaluation of hypoxia-induced astrocytic commitment of neuroepithelial stem cells.